Hotspot

rps8-rpl14

LSC · 84471-84662 · 192 bp

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Pi 0.0273
MarkerSeek score 72.10
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
192
variable_sites
5
pi
0.0273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TTCCGCGATGTTAGTGGATG GAACTCGAGTTTTTGGTGCG 50.0 50.0 58.1 58.0 368-377 bp 1.0 82.3
rps8-rpl14_p2 TTCCGCGATGTTAGTGGATG CGATTGCCCGAGAATTGAGA 50.0 50.0 58.1 58.1 350-359 bp 1.0 82.2
rps8-rpl14_p3 TTCCGCGATGTTAGTGGATG TTGCCCGAGAATTGAGACAG 50.0 50.0 58.1 57.9 347-356 bp 1.0 81.9
rps8-rpl14_p4 CGCGATGTTAGTGGATGCTA GAACTCGAGTTTTTGGTGCG 50.0 50.0 57.9 58.0 365-374 bp 1.0 81.8
rps8-rpl14_p5 TTCCGCGATGTTAGTGGATG ACTCGAGTTTTTGGTGCGAT 50.0 45.0 58.1 58.1 366-375 bp 1.0 81.5