Hotspot

psbT-psbN

LSC · 78274-78338 · 65 bp

Back to Plinia

Pi 0.0308
MarkerSeek score 70.20
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
65
variable_sites
2
pi
0.0308
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 CGAGAACCACCTAAAGTTCCA ATCCATTCGAAGAACACGGG 47.6 50.0 57.6 58.0 130 bp 1.0 69.9
psbT-psbN_p2 CGAGAACCACCTAAAGTTCCA CCCTGGTCGCCATCTCTATA 47.6 55.0 57.6 58.1 223 bp 1.0 68.9
psbT-psbN_p3 TCGAGAACCACCTAAAGTTCC ATCCATTCGAAGAACACGGG 47.6 50.0 57.3 58.0 131 bp 1.0 67.6
psbT-psbN_p4 CGAGAACCACCTAAAGTTCCA TTCGAAGAACACGGGGACTA 47.6 50.0 57.6 58.4 125 bp 1.0 67.1
psbT-psbN_p5 TCGAGAACCACCTAAAGTTCC CCCTGGTCGCCATCTCTATA 47.6 55.0 57.3 58.1 224 bp 1.0 66.7