Hotspot

clpP-psbB

LSC · 76128-76532 · 405 bp

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Pi 0.0204
MarkerSeek score 58.20
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
405
variable_sites
8
pi
0.0204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 488-501 bp 1.0 81.5
clpP-psbB_p2 CGCACTATACGTCAACCCAA ACACGATACCAAGGCAAACC 50.0 50.0 58.0 58.5 491-504 bp 1.0 79.6
clpP-psbB_p3 TCCTCTCCAGGACTTCGAAA CGATACCAAGGCAAACCCAT 50.0 50.0 57.7 58.2 459-472 bp 1.0 79.3
clpP-psbB_p4 TCCAGGACTTCGAAAGGGTA CGATACCAAGGCAAACCCAT 50.0 50.0 57.7 58.2 454-467 bp 1.0 79.2
clpP-psbB_p5 CGCACTATACGTCAACCCAA TGAACACGATACCAAGGCAA 50.0 45.0 58.0 57.4 494-507 bp 1.0 78.9