Hotspot

petA-psbJ

LSC · 66861-67853 · 993 bp

Back to Plinia

Pi 0.0101
MarkerSeek score 64.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
993
variable_sites
10
pi
0.0101
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC ATACTCCGGGTTGGGTTCAT 50.0 50.0 57.8 58.4 1122-1123 bp 1.0 76.4
petA-psbJ_p2 GATCCATTCCGTGTCCAAGG ATACTCCGGGTTGGGTTCAT 55.0 50.0 58.3 58.4 1140-1141 bp 1.0 74.9
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC TTCATACTCCGGGTTGGGTT 50.0 50.0 57.8 58.6 1125-1126 bp 1.0 74.5
petA-psbJ_p4 TGTCCAAGGCCTTTTGTTCT ATACTCCGGGTTGGGTTCAT 45.0 50.0 57.5 58.4 1129-1130 bp 1.0 74.0
petA-psbJ_p5 GGCCTTTTGTTCTTCTTGGC ATGGTTCATACTCCGGGTTG 50.0 50.0 57.8 57.3 1129-1130 bp 1.0 73.9