Hotspot

ndhC-trnV-UAC

LSC · 54257-54766 · 510 bp

Back to Plinia

Pi 0.0179
MarkerSeek score 64.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
510
variable_sites
9
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AATCGGGGCTAAAATTCCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 637-645 bp 1.0 78.0
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 645-653 bp 1.0 77.8
ndhC-trnV-UAC_p3 AATCGGGGCTAAAATTCCGG AAGGCATGTTGGGTCTTTGA 50.0 45.0 58.3 57.6 704-712 bp 1.0 76.4
ndhC-trnV-UAC_p4 AATCGGGGCTAAAATTCCGG AGGCATGTTGGGTCTTTGAA 50.0 45.0 58.3 57.6 703-711 bp 1.0 76.4
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA AGGCATGTTGGGTCTTTGAA 50.0 45.0 57.7 57.6 711-719 bp 1.0 76.1