Hotspot

ycf3-trnS-GGA

LSC · 47748-48580 · 833 bp

Back to Plinia

Pi 0.00723
MarkerSeek score 63.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
833
variable_sites
6
pi
0.00723
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 994-996 bp 1.0 67.3
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 995-997 bp 1.0 67.3
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1015-1017 bp 1.0 66.8
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1016-1018 bp 1.0 66.8
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 965-967 bp 1.0 66.5