Hotspot

ycf1

SSC · 90873-95891 · 5019 bp

Back to Plicosepalus

Pi 0.0547

MarkerSeek score 70.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5019
variable_sites: 268
pi: 0.0547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTTTGGTCCTCCCCGAAAA	ACGTTTCATGATAGTGAAGGACA	50.0	39.1	58.0	57.9	783 bp	0.5	34.7
ycf1_p2	TTTGGTCCTCCCCGAAAAAG	ACGTTTCATGATAGTGAAGGACA	50.0	39.1	58.0	57.9	781 bp	0.5	34.7
ycf1_p3	GGGAATTTCCGCAAAACTGG	ACGTTTCATGATAGTGAAGGACA	50.0	39.1	57.9	57.9	969 bp	0.5	34.2
ycf1_p4	GCTGAAATGCCTTTTGGTCC	ACGTTTCATGATAGTGAAGGACA	50.0	39.1	57.9	57.9	793 bp	0.5	34.1
ycf1_p5	ATCCAAAGGCGTAAAACGGT	ACGTTTCATGATAGTGAAGGACA	45.0	39.1	58.1	57.9	1441 bp	0.5	34.0