Hotspot

rpl16

LSC · 66820-67617 · 798 bp

Back to Plicosepalus

Pi 0.106

MarkerSeek score 83.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 798
variable_sites: 79
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GTTGCGTCTTTGATCACAGC	ATCGAGTGGATCAGAGAGGG	50.0	55.0	58.0	58.0	1267-1661 bp	1.0	88.1
rpl16_p2	GTTGCGTCTTTGATCACAGC	GTCGGATCGACGGAAAAGAA	50.0	50.0	58.0	58.0	1296-1690 bp	1.0	87.9
rpl16_p3	TCTGGATCAACGACAACTGC	ATCGAGTGGATCAGAGAGGG	50.0	55.0	58.2	58.0	1396-1790 bp	1.0	86.2
rpl16_p4	TCTGGATCAACGACAACTGC	GTCGGATCGACGGAAAAGAA	50.0	50.0	58.2	58.0	1425-1819 bp	1.0	86.0
rpl16_p5	GTTGCGTCTTTGATCACAGC	GTCGACGGTATTGCGTGTAT	50.0	50.0	58.0	58.2	1624-2018 bp	1.0	85.5