Hotspot

ccsA

SSC · 96743-97684 · 942 bp

Back to Plicosepalus

Pi 0.0572

MarkerSeek score 78.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 942
variable_sites: 51
pi: 0.0572
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGAGGACTGTGTCGGTTGTA	ACGTTGCTCTTAGGAAGCAG	50.0	50.0	58.0	57.9	1409-1626 bp	1.0	88.4
ccsA_p2	AGAGGACTGTGTCGGTTGTA	CGTTGCTCTTAGGAAGCAGT	50.0	50.0	58.0	57.9	1408-1625 bp	1.0	88.4
ccsA_p3	AGAGGACTGTGTCGGTTGTA	GGGGCTCTGGACAAAATTGA	50.0	50.0	58.0	58.1	1532-1737 bp	1.0	88.3
ccsA_p4	CAAATTGCTTCCGCTCCAAG	GGGGCTCTGGACAAAATTGA	50.0	50.0	58.0	58.1	1555-1760 bp	1.0	88.3
ccsA_p5	CAAATTGCTTCCGCTCCAAG	CGTTGCTCTTAGGAAGCAGT	50.0	50.0	58.0	57.9	1431-1648 bp	1.0	88.3