Hotspot

trnS-GGA-rps4

LSC · 38184-38330 · 147 bp

Back to Plicosepalus

Pi 0.0634

MarkerSeek score 80.20

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 9
pi: 0.0634
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	CACTCCGTTTTGGGTTCAGA	AGTAAATCGGTCGGTTTGCA	50.0	45.0	58.0	57.8	332-350 bp	1.0	81.6
trnS-GGA-rps4_p2	TCACTCCGTTTTGGGTTCAG	AGTAAATCGGTCGGTTTGCA	50.0	45.0	58.0	57.8	333-351 bp	1.0	81.6
trnS-GGA-rps4_p3	GGCTTTTTGTTTACCGAGGG	AGTAAATCGGTCGGTTTGCA	50.0	45.0	57.3	57.8	253-271 bp	1.0	77.2
trnS-GGA-rps4_p4	TTACGTAGGAGAGATGGCCG	AGTAAATCGGTCGGTTTGCA	55.0	45.0	58.7	57.8	308-326 bp	1.0	76.7
trnS-GGA-rps4_p5	ACTCCGTTTTGGGTTCAGAA	AGTAAATCGGTCGGTTTGCA	45.0	45.0	57.3	57.8	331-349 bp	1.0	76.1