Hotspot

psbM-trnD-GUC

LSC · 23681-24304 · 624 bp

Back to Plicosepalus

Pi 0.0681

MarkerSeek score 78.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 41
pi: 0.0681
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATCGCTTGGGCTGCTTTAAT	GAACCGATGACTTACGCCTT	45.0	50.0	58.2	58.0	1987-2536 bp	1.0	85.3
psbM-trnD-GUC_p2	ATCGCTTGGGCTGCTTTAAT	CCATGGCGTTACTCTACCAC	45.0	55.0	58.2	58.1	1966-2515 bp	1.0	84.9
psbM-trnD-GUC_p3	ATCGCTTGGGCTGCTTTAAT	TGGCGTTACTCTACCACTGA	45.0	50.0	58.2	58.1	1963-2512 bp	1.0	84.7
psbM-trnD-GUC_p4	GTATCGCTTGGGCTGCTTTA	GAACCGATGACTTACGCCTT	50.0	50.0	58.3	58.0	1989-2538 bp	1.0	84.4
psbM-trnD-GUC_p5	ATCGCTTGGGCTGCTTTAAT	CCGATGACTTACGCCTTACC	45.0	55.0	58.2	58.2	1984-2533 bp	1.0	84.1