Hotspot

petN-psbM

LSC · 23076-23566 · 491 bp

Back to Plicosepalus

Pi 0.0457

MarkerSeek score 76.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 19
pi: 0.0457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATCGCTTGGGCTGCTTTAAT	ACGTAAAAACGGCTAGTCAA	45.0	40.0	58.2	55.4	562-604 bp	1.0	63.6
petN-psbM_p2	ATCGCTTGGGCTGCTTTAAT	TCCTTTACGTAAAAACGGCT	45.0	40.0	58.2	55.3	568-610 bp	1.0	63.3
petN-psbM_p3	GTATCGCTTGGGCTGCTTTA	ACGTAAAAACGGCTAGTCAA	50.0	40.0	58.3	55.4	564-606 bp	1.0	62.7
petN-psbM_p4	GTATCGCTTGGGCTGCTTTA	TCCTTTACGTAAAAACGGCT	50.0	40.0	58.3	55.3	570-612 bp	1.0	62.4
petN-psbM_p5	TATCGCTTGGGCTGCTTTAA	ACGTAAAAACGGCTAGTCAA	45.0	40.0	57.6	55.4	563-605 bp	1.0	62.1