Hotspot

rpoB-trnC-GCA

LSC · 21815-22657 · 843 bp

Back to Plicosepalus

Pi 0.0988

MarkerSeek score 75.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 60
pi: 0.0988
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATTTCCATCCCAGAGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	56.6	58.1	717-925 bp	1.0	77.7
rpoB-trnC-GCA_p2	TCATTTCCATCCCAGAGCAT	AACTGGGGAAAGGGGATTTG	45.0	50.0	56.6	57.7	708-916 bp	1.0	75.8
rpoB-trnC-GCA_p3	TCATTTCCATCCCAGAGCAT	ATTTGAACTGGGGAAAGGGG	45.0	50.0	56.6	57.7	713-921 bp	1.0	75.8
rpoB-trnC-GCA_p4	TCATTTCCATCCCAGAGCAT	TGAACTGGGGAAAGGGGATT	45.0	50.0	56.6	58.5	710-918 bp	1.0	74.1
rpoB-trnC-GCA_p5	TCATTTCCATCCCAGAGCAT	TTGAACTGGGGAAAGGGGAT	45.0	50.0	56.6	58.5	711-919 bp	1.0	74.1