Hotspot

ycf66

Genome · 4202-5125 · 924 bp

Back to Pleurozia

Pi 0.0231

MarkerSeek score 59.10

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 924
variable_sites: 31
pi: 0.0231
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	AAGCATGGAGGTTCGAATCC	AAGTGGTAAGGCAGAGGACT	50.0	50.0	58.0	58.0	1946-1959 bp	1.0	82.7
ycf66_p2	GCCTTGGTGGTGAAATGGTA	AAGTGGTAAGGCAGAGGACT	50.0	50.0	58.1	58.0	1995-2008 bp	1.0	82.3
ycf66_p3	AAGCATGGAGGTTCGAATCC	GTGACATGGCCAAGTGGTAA	50.0	50.0	58.0	58.1	1957-1970 bp	1.0	82.2
ycf66_p4	AAGCATGGAGGTTCGAATCC	CAAGTGGTAAGGCAGAGGAC	50.0	55.0	58.0	57.9	1947-1960 bp	1.0	82.2
ycf66_p5	ATGTGCAAACCGCTAGTCAA	AAGTGGTAAGGCAGAGGACT	45.0	50.0	58.1	58.0	1611 bp	0.333333	52.2