Hotspot

ndhF

Genome · 89519-91612 · 2094 bp

Back to Pleurozia

Pi 0.0239

MarkerSeek score 58.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2094
variable_sites: 74
pi: 0.0239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCCGCAACCGATTTACTAA	CAGGTTCTACTCGGAGTTGC	45.0	55.0	57.9	58.0	2631-2641 bp	1.0	82.6
ndhF_p2	TGCCGCAACCGATTTACTAA	GGTTCTACTCGGAGTTGCTG	45.0	55.0	57.9	58.0	2629-2639 bp	1.0	82.6
ndhF_p3	TGCCGCAACCGATTTACTAA	ACCCTTAACTATGGCACCCT	45.0	50.0	57.9	58.0	2783-2793 bp	1.0	82.4
ndhF_p4	TGCCGCAACCGATTTACTAA	CTTTAACCACGGGTGTCCAA	45.0	50.0	57.9	58.0	2765-2775 bp	1.0	82.3
ndhF_p5	TACGAGACAAAGGGAGGAGG	GGTTCTACTCGGAGTTGCTG	55.0	55.0	58.2	58.0	2713-2723 bp	1.0	82.1