Hotspot

rps19-rpl2

Genome · 78804-78841 · 38 bp

Back to Pleurozia

Pi 0.0702

MarkerSeek score 79.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 38
variable_sites: 4
pi: 0.0702
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGTAGTTGCACGAGACCATG	ATGGAGGAGGTGAGGGAAAA	50.0	50.0	57.9	57.9	290-293 bp	1.0	82.2
rps19-rpl2_p2	GTAGTTGCACGAGACCATGT	ATGGAGGAGGTGAGGGAAAA	50.0	50.0	57.9	57.9	289-292 bp	1.0	82.2
rps19-rpl2_p3	TGGGTCACATGCACGATATG	ATGGAGGAGGTGAGGGAAAA	50.0	50.0	58.0	57.9	628-631 bp	1.0	81.8
rps19-rpl2_p4	TGGGTCACATGCACGATATG	AAGCAGGGTCTAAACGTTGG	50.0	50.0	58.0	58.1	703-706 bp	1.0	81.4
rps19-rpl2_p5	TTGAGCTCTAGGCCGAAAAC	ATGGAGGAGGTGAGGGAAAA	50.0	50.0	57.9	57.9	760-763 bp	1.0	81.2