Hotspot

rpl14-rpl16

Genome · 76209-76336 · 128 bp

Back to Pleurozia

Pi 0.0703

MarkerSeek score 77.60

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 128
variable_sites: 13
pi: 0.0703
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TGGGACCAAAAACACGAGTT	GCTCGTCGAGGTGGTAAATT	45.0	50.0	57.9	58.0	652-676 bp	1.0	84.2
rpl14-rpl16_p2	TGGGACCAAAAACACGAGTT	ACGTCCCGACAAATAGAAGC	45.0	50.0	57.9	58.0	697-721 bp	1.0	83.8
rpl14-rpl16_p3	TGGGACCAAAAACACGAGTT	CCGACAAATAGAAGCGGGAA	45.0	50.0	57.9	58.0	692-716 bp	1.0	83.8
rpl14-rpl16_p4	CGAGTTCCTTTGGGGTTACC	GCTCGTCGAGGTGGTAAATT	55.0	50.0	58.2	58.0	638-662 bp	1.0	83.8
rpl14-rpl16_p5	CGAGTTCCTTTGGGGTTACC	ACGTCCCGACAAATAGAAGC	55.0	50.0	58.2	58.0	683-707 bp	1.0	83.4