Hotspot

psaJ-rpl33

Genome · 64604-64617 · 14 bp

Back to Pleurozia

Pi 0.143
MarkerSeek score 78.60
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
Genome
length_bp
14
variable_sites
3
pi
0.143
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TGTGGTTCGGATTTTTAGCC TCCATAGTTTTGAGCACAATCA 45.0 36.4 56.3 56.0 163 bp 1.0 41.5
psaJ-rpl33_p2 TTGTGGTTCGGATTTTTAGCC TCCATAGTTTTGAGCACAATCA 42.9 36.4 57.1 56.0 164 bp 1.0 41.5
psaJ-rpl33_p3 TGTGGTTCGGATTTTTAGCC TCCATAGTTTTGAGCACAATCAA 45.0 34.8 56.3 56.7 163 bp 1.0 41.5
psaJ-rpl33_p4 TGTGGTTCGGATTTTTAGCC TTCCATAGTTTTGAGCACAATCA 45.0 34.8 56.3 56.7 164 bp 1.0 41.5
psaJ-rpl33_p5 TGTGGTTCGGATTTTTAGCC CCATAGTTTTGAGCACAATCA 45.0 38.1 56.3 54.5 162 bp 1.0 41.5