Hotspot

trnM-CAU-atpE

Genome · 53589-53649 · 61 bp

Back to Pleurozia

Pi 0.0656

MarkerSeek score 77.70

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: Genome
length_bp: 61
variable_sites: 6
pi: 0.0656
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCGAGAGTCATTGGTTCAA	GAGCTAAAGCAAGATTAGAAGCA	50.0	39.1	58.2	56.9	152 bp	1.0	49.1
trnM-CAU-atpE_p2	GGCGAGAGTCATTGGTTCAA	AGCTAAAGCAAGATTAGAAGCA	50.0	36.4	58.2	55.8	151 bp	1.0	48.1
trnM-CAU-atpE_p3	GGCGAGAGTCATTGGTTCAA	AGAGCTAAAGCAAGATTAGAAGCA	50.0	37.5	58.2	58.3	153 bp	1.0	47.5
trnM-CAU-atpE_p4	GGCGAGAGTCATTGGTTCAA	AGAGCTAAAGCAAGATTAGAAGC	50.0	39.1	58.2	56.7	153 bp	1.0	47.0
trnM-CAU-atpE_p5	TCATACGGCGAGAGTCATTG	GAGCTAAAGCAAGATTAGAAGCA	50.0	39.1	57.5	56.9	158 bp	1.0	46.6