Hotspot

psbZ-trnG-GCC

Genome · 41355-41578 · 224 bp

Back to Pleurozia

Pi 0.0551

MarkerSeek score 76.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: Genome
length_bp: 224
variable_sites: 18
pi: 0.0551
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TAGGTCATTTATGGCACGCC	CGGAGTAGAGCAGTCTGGTA	50.0	55.0	58.0	58.0	1010-1025 bp	1.0	83.7
psbZ-trnG-GCC_p2	TAGGTCATTTATGGCACGCC	GTTGGTAAGGATCGCGGATT	50.0	50.0	58.0	58.1	1109-1125 bp	1.0	83.4
psbZ-trnG-GCC_p3	TAGGTCATTTATGGCACGCC	AGTCGTCTTCATCGTCGTTG	50.0	50.0	58.0	58.0	1236-1252 bp	1.0	83.3
psbZ-trnG-GCC_p4	TAGGTCATTTATGGCACGCC	CAAGTCGTCTTCATCGTCGT	50.0	50.0	58.0	58.0	1238-1254 bp	1.0	83.3
psbZ-trnG-GCC_p5	CGCTCCTCTAGGGTCACTAA	AGTCGTCTTCATCGTCGTTG	55.0	50.0	57.9	58.0	1358-1374 bp	1.0	82.9