Hotspot

psbC-trnS-UGA

Genome · 40856-40972 · 117 bp

Back to Pleurozia

Pi 0.0684

MarkerSeek score 79.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: Genome
length_bp: 117
variable_sites: 12
pi: 0.0684
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	ACTTTCTATGACTCCTCTTAATT	TTCGAATCCCTCTCTCTCCT	30.4	50.0	52.6	56.9	162 bp	1.0	48.4
psbC-trnS-UGA_p2	ACTTTCTATGACTCCTCTTAATT	GGTTCGAATCCCTCTCTCTC	30.4	55.0	52.6	56.8	164 bp	1.0	48.3
psbC-trnS-UGA_p3	ACTTTCTATGACTCCTCTTAATT	GTTCGAATCCCTCTCTCTCC	30.4	55.0	52.6	56.8	163 bp	1.0	48.3
psbC-trnS-UGA_p4	ACTTTCTATGACTCCTCTTAATT	GGGTTCGAATCCCTCTCTCT	30.4	55.0	52.6	58.2	165 bp	1.0	48.2
psbC-trnS-UGA_p5	ACTTTCTATGACTCCTCTTAATTA	GGGTTCGAATCCCTCTCTCT	29.2	55.0	52.7	58.2	165 bp	1.0	48.2