Hotspot

psbI-psbK

Genome · 23368-23698 · 331 bp

Back to Pleurozia

Pi 0.0423

MarkerSeek score 70.30

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: Genome
length_bp: 331
variable_sites: 19
pi: 0.0423
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	CCAAAAACAAAAAGGGAAACA	TTTGTTTGGCAAGCTTCTGT	33.3	40.0	53.8	56.7	422-436 bp	1.0	45.4
psbI-psbK_p2	CCAAAAACAAAAAGGGAAACAA	TTTGTTTGGCAAGCTTCTGT	31.8	40.0	54.7	56.7	422-436 bp	1.0	45.4
psbI-psbK_p3	CCAAAAACAAAAAGGGAAACAAA	TTTGTTTGGCAAGCTTCTGT	30.4	40.0	55.4	56.7	422-436 bp	1.0	45.4
psbI-psbK_p4	CCAAAAACAAAAAGGGAAACA	TGTTTGGCAAGCTTCTGTAAG	33.3	42.9	53.8	57.0	420-434 bp	1.0	45.4
psbI-psbK_p5	CCAAAAACAAAAAGGGAAACA	GTTTGGCAAGCTTCTGTAAGT	33.3	42.9	53.8	57.0	419-433 bp	1.0	45.4