Hotspot

atpI-atpH

Genome · 17805-18075 · 271 bp

Back to Pleurozia

Pi 0.0498

MarkerSeek score 77.10

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 271
variable_sites: 19
pi: 0.0498
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	AGGAGAATCTATGGAGGGTCA	GCAGCAATAACAGAAGCAGC	47.6	50.0	57.0	58.1	327-348 bp	1.0	71.1
atpI-atpH_p2	AGGAGAATCTATGGAGGGTCA	AGCAGCAGAAATCAAAGGGT	47.6	45.0	57.0	57.7	313-334 bp	1.0	69.8
atpI-atpH_p3	AGGAGAATCTATGGAGGGTCA	CCAGCAGCAATAACAGAAGC	47.6	50.0	57.0	57.4	330-351 bp	1.0	67.3
atpI-atpH_p4	AGGAGAATCTATGGAGGGTCAT	GCAGCAATAACAGAAGCAGC	45.5	50.0	57.5	58.1	327-348 bp	1.0	67.1
atpI-atpH_p5	GGAGAATCTATGGAGGGTCA	GCAGCAATAACAGAAGCAGC	50.0	50.0	55.4	58.1	326-347 bp	1.0	66.3