Hotspot

rpl2-rpl23

IRb · 87533-87550 · 18 bp

Back to Pleurothallis

Pi 0.111

MarkerSeek score 87.50

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 18
variable_sites: 2
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CACTTGACTGTCTACGGCTC	CGCTTCAACCGGGTTATTCT	55.0	50.0	58.0	58.3	134 bp	1.0	78.9
rpl2-rpl23_p2	CCACAACGATGCTGTCCATA	CGCTTCAACCGGGTTATTCT	50.0	50.0	58.0	58.3	181 bp	1.0	78.6
rpl2-rpl23_p3	GACTGTCTACGGCTCCATTG	CGCTTCAACCGGGTTATTCT	55.0	50.0	58.1	58.3	129 bp	1.0	78.5
rpl2-rpl23_p4	CACTTGACTGTCTACGGCTC	ATCATTACGCTTCAACCGGG	55.0	50.0	58.0	58.3	141 bp	1.0	78.3
rpl2-rpl23_p5	CCACAACGATGCTGTCCATA	ATCATTACGCTTCAACCGGG	50.0	50.0	58.0	58.3	188 bp	1.0	78.0