Hotspot

clpP1-psbB

LSC · 72292-73116 · 825 bp

Back to Pleurothallis

Pi 0.0308

MarkerSeek score 66.90

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 20
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGCACTATACGTCAACCCAA	ACAGAAAGTAATCGACCGGG	50.0	50.0	58.0	57.3	790-966 bp	1.0	80.5
clpP1-psbB_p2	TATACGTCAACCCAAGCTGC	ACAGAAAGTAATCGACCGGG	50.0	50.0	58.0	57.3	785-961 bp	1.0	80.3
clpP1-psbB_p3	CTTTTGGAACACCAATGGGC	ACAGAAAGTAATCGACCGGG	50.0	50.0	57.8	57.3	736-912 bp	1.0	79.3
clpP1-psbB_p4	TTTGGAACACCAATGGGCAT	ACAGAAAGTAATCGACCGGG	45.0	50.0	58.3	57.3	734-910 bp	1.0	78.4
clpP1-psbB_p5	CGCACTATACGTCAACCCAA	CAGAAAGTAATCGACCGGGA	50.0	50.0	58.0	57.0	789-965 bp	1.0	78.2