Hotspot

cemA-petA

LSC · 61795-62036 · 242 bp

Back to Pleurothallis

Pi 0.0426
MarkerSeek score 79.20
Primer available yes
Label cemA-petA

Feature Metadata

label_name
cemA-petA
feature_type
igs
region
LSC
length_bp
242
variable_sites
10
pi
0.0426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-petA_p1 CGTGTATCTCCTTCGCTTGT TGGATCGAGTCATCTGTTCT 50.0 45.0 57.7 55.7 335-342 bp 1.0 63.3
cemA-petA_p2 CGTGTATCTCCTTCGCTTGT TGGATCGAGTCATCTGTTCTT 50.0 42.9 57.7 56.5 335-342 bp 1.0 61.6
cemA-petA_p3 TCGTGTATCTCCTTCGCTTG TGGATCGAGTCATCTGTTCT 50.0 45.0 57.4 55.7 336-343 bp 1.0 61.0
cemA-petA_p4 CGTGTATCTCCTTCGCTTGT ATGGATCGAGTCATCTGTTCT 50.0 42.9 57.7 56.3 336-343 bp 1.0 59.8
cemA-petA_p5 CGTGTATCTCCTTCGCTTGT TGGATCGAGTCATCTGTTCTTT 50.0 40.9 57.7 57.2 335-342 bp 1.0 59.3