Hotspot

atpB-rbcL

LSC · 53483-54507 · 1025 bp

Back to Pleurothallis

Pi 0.0243

MarkerSeek score 66.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 23
pi: 0.0243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACAGGATCAGAAGTAGTGGGA	ACCAGCTTTAAATCCAACGC	47.6	45.0	57.5	57.0	1036-1105 bp	1.0	65.8
atpB-rbcL_p2	ACAGGATCAGAAGTAGTGGGA	AGCTTTAAATCCAACGCTTGC	47.6	42.9	57.5	58.1	1033-1102 bp	1.0	65.5
atpB-rbcL_p3	ACAGGATCAGAAGTAGTGGGA	GCTTTAAATCCAACGCTTGCT	47.6	42.9	57.5	58.1	1032-1101 bp	1.0	65.5
atpB-rbcL_p4	CACAGGATCAGAAGTAGTGGG	ACCAGCTTTAAATCCAACGC	52.4	45.0	57.5	57.0	1037-1106 bp	1.0	65.4
atpB-rbcL_p5	CCACAGGATCAGAAGTAGTGG	ACCAGCTTTAAATCCAACGC	52.4	45.0	57.5	57.0	1038-1107 bp	1.0	65.4