Hotspot

ndhC-trnV-UAC

LSC · 49815-50502 · 688 bp

Back to Pleurothallis

Pi 0.033

MarkerSeek score 71.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 22
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCTGGACCTTCCCTAATCGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	826-837 bp	1.0	81.0
ndhC-trnV-UAC_p2	TCTGGACCTTCCCTAATCGG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.2	57.5	825-836 bp	1.0	78.2
ndhC-trnV-UAC_p3	TCTGGACCTTCCCTAATCGG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.2	58.7	814-825 bp	1.0	77.1
ndhC-trnV-UAC_p4	TCTGGACCTTCCCTAATCGG	AAGGTCTACGGTTCGAGTCC	55.0	55.0	58.2	58.8	824-835 bp	1.0	75.9
ndhC-trnV-UAC_p5	TCTGGACCTTCCCTAATCGG	GAGAAGGTCTACGGTTCGAG	55.0	55.0	58.2	57.2	827-838 bp	1.0	75.9