Hotspot

trnE-UUC-trnT-GGU

LSC · 31413-32065 · 653 bp

Back to Pleurothallis

Pi 0.0448

MarkerSeek score 72.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 29
pi: 0.0448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	ACCACTGAGTTAAAAGGGCC	50.0	50.0	57.8	57.7	723-726 bp	1.0	80.2
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	ACCACTGAGTTAAAAGGGCC	50.0	50.0	57.7	57.7	701-704 bp	1.0	79.4
trnE-UUC-trnT-GGU_p3	TTTCGTAGTCCACTACCCCC	ACCACTGAGTTAAAAGGGCC	55.0	50.0	58.4	57.7	760-763 bp	1.0	77.9
trnE-UUC-trnT-GGU_p4	CCGTTGCCTCCTTGAAAGAG	ACCACTGAGTTAAAAGGGCC	55.0	50.0	58.8	57.7	724-727 bp	1.0	74.8
trnE-UUC-trnT-GGU_p5	GAACCACTAGACGATGAGGG	ACCACTGAGTTAAAAGGGCC	55.0	50.0	57.1	57.7	695-698 bp	1.0	74.7