Hotspot

psbK-psbI

LSC · 7045-7574 · 530 bp

Back to Pleurothallis

Pi 0.0517

MarkerSeek score 84.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 26
pi: 0.0517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTGTTTGGCAAGCTGCTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	645-654 bp	1.0	85.5
psbK-psbI_p2	CTTTGTTTGGCAAGCTGCTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	646-655 bp	1.0	85.5
psbK-psbI_p3	AATCGTAGACGTTATGCCGG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	693-702 bp	1.0	84.5
psbK-psbI_p4	AATCGTAGACGTTATGCCGG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	694-703 bp	1.0	84.5
psbK-psbI_p5	CGTTATGCCGGTTATCCCTT	ACGCCCCGGATCATTAGATA	50.0	50.0	57.8	58.1	684-693 bp	1.0	83.6