Hotspot

trnK-UUU-rps16

LSC · 4258-4838 · 581 bp

Back to Pleurothallis

Pi 0.0314
MarkerSeek score 73.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
581
variable_sites
18
pi
0.0314
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 674-679 bp 1.0 68.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 673-678 bp 1.0 68.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 673-678 bp 1.0 67.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 672-677 bp 1.0 67.5
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GTGCTCAACCCACAGGAAC 50.0 57.9 57.5 58.7 660-665 bp 1.0 66.0