Hotspot

matK-trnK-UUU

LSC · 3339-4220 · 882 bp

Back to Pleurothallis

Pi 0.0336

MarkerSeek score 70.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 29
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTGAAGGAGTAGCGGATA	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.0	57.9	1015-1203 bp	1.0	74.2
matK-trnK-UUU_p2	TCCTGAAGGAGTAGCGGATA	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.0	58.2	998-1186 bp	1.0	73.7
matK-trnK-UUU_p3	TCCTGAAGGAGTAGCGGATA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.0	58.2	997-1185 bp	1.0	73.7
matK-trnK-UUU_p4	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	50.0	50.0	57.0	57.6	1016-1204 bp	1.0	71.9
matK-trnK-UUU_p5	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	50.0	50.0	57.0	57.6	1018-1206 bp	1.0	71.9