Hotspot

ycf1

SSC · 126137-131581 · 5445 bp

Back to Pleurospermum

Pi 0.0129

MarkerSeek score 49.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 212
pi: 0.0129
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGCTTGGGGATATGGAACG	CAAACGTAGTCGCAAAGCAG	50.0	50.0	57.9	58.1	377 bp	1.0	84.6
ycf1_p2	TTGCTTGGGGATATGGAACG	CGCAAAGCAGGTGTCAAAAA	50.0	45.0	57.9	58.1	367 bp	1.0	84.6
ycf1_p3	TTGCTTGGGGATATGGAACG	CCTACCAAACGTAGTCGCAA	50.0	50.0	57.9	57.9	382 bp	1.0	84.5
ycf1_p4	AAAGGAAGGTTTGTTTGCGC	CAAACGTAGTCGCAAAGCAG	45.0	50.0	58.1	58.1	744 bp	0.375	54.8
ycf1_p5	AAAGGAAGGTTTGTTTGCGC	CCTACCAAACGTAGTCGCAA	45.0	50.0	58.1	57.9	749 bp	0.375	54.8