Hotspot

ndhH-rps15

SSC · 125373-125463 · 91 bp

Back to Pleurospermum

Pi 0.0387

MarkerSeek score 70.50

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 91
variable_sites: 9
pi: 0.0387
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	GGTCATTTCGTGTAGCTGGT	AACGAAGACAACAAGGGGTC	50.0	50.0	57.9	58.0	345-350 bp	1.0	83.3
ndhH-rps15_p2	ATGAGTCGAAGAACACCGTG	AACGAAGACAACAAGGGGTC	50.0	50.0	57.9	58.0	403-408 bp	1.0	82.9
ndhH-rps15_p3	TGAGGTAAATCAGCAGCGAC	AACGAAGACAACAAGGGGTC	50.0	50.0	58.0	58.0	823-828 bp	1.0	82.2
ndhH-rps15_p4	CCCAAGCCATAACAAATGCG	AACGAAGACAACAAGGGGTC	50.0	50.0	58.1	58.0	674-679 bp	1.0	81.7
ndhH-rps15_p5	CCTAACTGCTCTGGTGCATT	AACGAAGACAACAAGGGGTC	50.0	50.0	57.9	58.0	586-591 bp	1.0	81.5