Hotspot

rpl32-trnL-UAG

SSC · 115534-116453 · 920 bp

Back to Pleurospermum

Pi 0.0183
MarkerSeek score 61.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
920
variable_sites
42
pi
0.0183
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGCAACGGGTTCTTAAAGG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1254-1563 bp 1.0 87.4
rpl32-trnL-UAG_p2 AGGCAACGGGTTCTTAAAGG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1255-1564 bp 1.0 87.3
rpl32-trnL-UAG_p3 GGGTATTGGGCAGCCTTAAA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 732-1045 bp 1.0 86.6
rpl32-trnL-UAG_p4 GGGTATTGGGCAGCCTTAAA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 733-1046 bp 1.0 86.6
rpl32-trnL-UAG_p5 AGGCAACGGGTTCTTAAAGG ACCAAAGGAAAAAGCCTCGT 50.0 45.0 58.1 57.9 1956-2265 bp 1.0 86.1