Hotspot

psbE-petL

LSC · 66201-67357 · 1157 bp

Back to Pleurospermum

Pi 0.0145

MarkerSeek score 65.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 48
pi: 0.0145
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1279-1303 bp	1.0	85.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1281-1305 bp	1.0	85.6
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1292-1316 bp	1.0	85.6
psbE-petL_p4	ACTGACGAATAACCAACCCG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1292-1316 bp	1.0	85.3
psbE-petL_p5	ACTGACGAATAACCAACCCG	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1294-1318 bp	1.0	85.3