Hotspot

petA-psbJ

LSC · 64389-65425 · 1037 bp

Back to Pleurospermum

Pi 0.0164

MarkerSeek score 70.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 53
pi: 0.0164
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1022-1223 bp	1.0	77.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1023-1224 bp	1.0	76.9
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	CTGGATTGGGTTCATCTCTGT	50.0	47.6	57.8	57.4	970-1171 bp	1.0	73.4
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGG	50.0	50.0	57.8	56.3	1022-1223 bp	1.0	72.8
petA-psbJ_p5	GCCTTTTGTTCTTCTTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.1	58.1	1021-1222 bp	1.0	71.7