Hotspot

trnF-GAA-ndhJ

LSC · 49802-50187 · 386 bp

Back to Pleurospermum

Pi 0.0281

MarkerSeek score 67.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 30
pi: 0.0281
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TAGCACCAGGTGGACTGTTA	55.0	50.0	58.0	58.0	751-776 bp	1.0	87.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TAGCACCAGGTGGACTGTTA	50.0	50.0	58.1	58.0	715-740 bp	1.0	87.3
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AAAACCCGAGGATTGGCATT	55.0	45.0	58.0	58.1	834-859 bp	1.0	86.3
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	930-955 bp	1.0	86.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.1	58.0	894-919 bp	1.0	85.9