Hotspot

petN-psbM

LSC · 29526-30616 · 1091 bp

Back to Pleurospermum

Pi 0.0218

MarkerSeek score 65.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 68
pi: 0.0218
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TTCCCGATCCCCCTCTATTC	AAGTCTCCCCTTCTGACTCC	55.0	55.0	58.0	58.1	2021-2088 bp	1.0	85.0
petN-psbM_p2	TTCCCGATCCCCCTCTATTC	CTTTCCCTTTTCGTTTCGCC	55.0	50.0	58.0	58.0	2299-2366 bp	0.875	79.6
petN-psbM_p3	CTTCCCGATCCCCCTCTATT	CTTTCCCTTTTCGTTTCGCC	55.0	50.0	58.0	58.0	2300-2367 bp	0.875	79.5
petN-psbM_p4	TTCCCGATCCCCCTCTATTC	CCCTTTTCGTTTCGCCTTTC	55.0	50.0	58.0	58.0	2295-2336 bp	0.75	73.4
petN-psbM_p5	CTTCCCGATCCCCCTCTATT	CCCTTTTCGTTTCGCCTTTC	55.0	50.0	58.0	58.0	2296-2337 bp	0.75	73.4