Hotspot

rpoB-trnC-GCA

LSC · 27547-28776 · 1230 bp

Back to Pleurospermum

Pi 0.021

MarkerSeek score 72.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 68
pi: 0.021
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCTCGGA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1242-1276 bp	1.0	86.3
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCTCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1282-1316 bp	1.0	85.9
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCTCGGA	TTATTGATCAGGCGACACCC	50.0	50.0	57.8	57.7	1300-1334 bp	1.0	83.9
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCTCGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1264-1298 bp	1.0	83.8
rpoB-trnC-GCA_p5	TCCCTCATTTCCATCTCGGA	CCTTACCACTCGGCCATATC	50.0	55.0	57.8	57.5	1243-1277 bp	1.0	83.1