Hotspot

trnK-UUU-rps16

LSC · 4238-5131 · 894 bp

Back to Pleurospermum

Pi 0.0295
MarkerSeek score 68.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
894
variable_sites
72
pi
0.0295
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG TGCTGCCATTTTGGAAGGAT 55.0 45.0 58.0 58.1 1688-1729 bp 1.0 84.9
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TGCTGCCATTTTGGAAGGAT 55.0 45.0 58.0 58.1 1687-1728 bp 1.0 84.9
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG AGGGATCCCAGAACAAGGAA 55.0 50.0 58.0 57.9 1619-1660 bp 1.0 84.9
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG AAGGGATCCCAGAACAAGGA 55.0 50.0 58.0 57.9 1619-1660 bp 1.0 84.9
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG AGGGATCCCAGAACAAGGAA 55.0 50.0 58.0 57.9 1618-1659 bp 1.0 84.9