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groEL

SSC · 13359-14948 · 1590 bp

Back to Pleurosigma

Pi 0.437

MarkerSeek score 80.60

Primer available yes

Label groEL

Feature Metadata

label_name: groEL
feature_type: gene
region: SSC
length_bp: 1590
variable_sites: 681
pi: 0.437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
groEL_p1	ACTTGTTTGACCTTAGCCACT	TGGCGACACCCCAAAATTTA	42.9	45.0	57.4	58.0	2153 bp	0.5	47.4
groEL_p2	ACTTTGGTACGGAATAAGCGA	TGGCGACACCCCAAAATTTA	42.9	45.0	57.4	58.0	2074 bp	0.5	47.2
groEL_p3	TGGTACGGAATAAGCGAAGA	TGGCGACACCCCAAAATTTA	45.0	45.0	56.4	58.0	2070 bp	0.5	47.0
groEL_p4	ACTTGTTTGACCTTAGCCACT	ACCTACGAATGGTGGAGTCA	42.9	50.0	57.4	58.1	2193 bp	0.5	46.8
groEL_p5	ACTTGTTTGACCTTAGCCACT	CGAACCTACGAATGGTGGAG	42.9	55.0	57.4	58.1	2196 bp	0.5	46.8