Hotspot

tRNA-Pro-atpA

IRb · 68735-69202 · 468 bp

Back to Pleurosigma

Pi 0.19

MarkerSeek score 78.10

Primer available yes

Label tRNA-Pro-atpA

Feature Metadata

label_name: tRNA-Pro-atpA
feature_type: igs
region: IRb
length_bp: 468
variable_sites: 87
pi: 0.19
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Pro-atpA_p1	AAAGCAGTTCGAACCGGAAT	ACAAAAACAGTTAGCGCGTG	45.0	45.0	58.1	57.9	1059 bp	0.5	58.3
tRNA-Pro-atpA_p2	AAAGCAGTTCGAACCGGAAT	ACACAAAAACAGTTAGCGCG	45.0	45.0	58.1	57.9	1061 bp	0.5	58.3
tRNA-Pro-atpA_p3	AAAGCAGTTCGAACCGGAAT	CACAAAAACAGTTAGCGCGT	45.0	45.0	58.1	57.9	1060 bp	0.5	58.3
tRNA-Pro-atpA_p4	AGGGGGCCAAAAAGTTTGAT	ACAAAAACAGTTAGCGCGTG	45.0	45.0	57.9	57.9	1216 bp	0.5	58.3
tRNA-Pro-atpA_p5	AGGGGGCCAAAAAGTTTGAT	CACAAAAACAGTTAGCGCGT	45.0	45.0	57.9	57.9	1217 bp	0.5	58.3