Hotspot

tRNA-Ala-tRNA-Ile

IRb · 65485-65486 · 2 bp

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Pi 0.5

MarkerSeek score 78.20

Primer available yes

Label tRNA-Ala-tRNA-Ile

Feature Metadata

label_name: tRNA-Ala-tRNA-Ile
feature_type: igs
region: IRb
length_bp: 2
variable_sites: 1
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ala-tRNA-Ile_p1	ATGTGGAGGTAAGCGGAGT	CCCTGATAAGGGTGAGGTCT	52.6	55.0	58.0	57.8	122 bp	1.0	71.1
tRNA-Ala-tRNA-Ile_p2	ATGTGGAGGTAAGCGGAGTC	CCCTGATAAGGGTGAGGTCT	55.0	55.0	59.2	57.8	122 bp	1.0	69.8
tRNA-Ala-tRNA-Ile_p3	ACCAACTGAGCTATACCCCC	CTCAGTTGGTTAGAGCGCAC	55.0	55.0	58.5	58.9	86 bp	1.0	69.6
tRNA-Ala-tRNA-Ile_p4	ACCAACTGAGCTATACCCCC	GGGCTATTAGCTCAGTTGGT	55.0	50.0	58.5	57.0	96 bp	1.0	68.8
tRNA-Ala-tRNA-Ile_p5	GAGGTAAGCGGAGTCGAAC	CCCTGATAAGGGTGAGGTCT	57.9	55.0	57.7	57.8	117 bp	1.0	68.6