Hotspot

tRNA-Gln-dnaK

SSC · 15314-15877 · 564 bp

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Pi 0.391

MarkerSeek score 79.30

Primer available yes

Label tRNA-Gln-dnaK

Feature Metadata

label_name: tRNA-Gln-dnaK
feature_type: igs
region: SSC
length_bp: 564
variable_sites: 218
pi: 0.391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gln-dnaK_p1	GCAGCTTGACGAATCAGAGA	GCAGGTATTTTAGCGGGTGA	50.0	50.0	58.0	58.0	1876 bp	0.5	59.7
tRNA-Gln-dnaK_p2	TAAATTTTGGGGTGTCGCCA	GCAGGTATTTTAGCGGGTGA	45.0	50.0	58.0	58.0	1360 bp	0.5	59.7
tRNA-Gln-dnaK_p3	AGCAGCTTGACGAATCAGAG	GCAGGTATTTTAGCGGGTGA	50.0	50.0	58.0	58.0	1877 bp	0.5	59.7
tRNA-Gln-dnaK_p4	GCAGCTTGACGAATCAGAGA	AGCAGGTATTTTAGCGGGTG	50.0	50.0	58.0	58.0	1877 bp	0.5	59.7
tRNA-Gln-dnaK_p5	TAAATTTTGGGGTGTCGCCA	AGCAGGTATTTTAGCGGGTG	45.0	50.0	58.0	58.0	1361 bp	0.5	59.7