Hotspot

ycf35-groEL

SSC · 12525-13358 · 834 bp

Back to Pleurosigma

Pi 0.413

MarkerSeek score 80.50

Primer available yes

Label ycf35-groEL

Feature Metadata

label_name: ycf35-groEL
feature_type: igs
region: SSC
length_bp: 834
variable_sites: 338
pi: 0.413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35-groEL_p1	AAAAGTCTCCGTCATGGCAG	AAGCTCGACGAGTTTTGGTT	50.0	45.0	58.2	58.1	2034 bp	0.5	58.1
ycf35-groEL_p2	CCGTCATGGCAGAGACTTTT	AAGCTCGACGAGTTTTGGTT	50.0	45.0	58.2	58.1	2026 bp	0.5	58.1
ycf35-groEL_p3	AAAAGTCTCCGTCATGGCAG	TGCGGGAATTAATGGTCCAG	50.0	50.0	58.2	57.9	1613 bp	0.5	58.1
ycf35-groEL_p4	CCGTCATGGCAGAGACTTTT	TGCGGGAATTAATGGTCCAG	50.0	50.0	58.2	57.9	1605 bp	0.5	58.1
ycf35-groEL_p5	CCGTCATGGCAGAGACTTTT	GCGGGAATTAATGGTCCAGT	50.0	50.0	58.2	57.9	1604 bp	0.5	58.1