Hotspot

psbA-ycf35

SSC · 8966-12128 · 3163 bp

Back to Pleurosigma

Pi 0.431

MarkerSeek score 78.00

Primer available yes

Label psbA-ycf35

Feature Metadata

label_name: psbA-ycf35
feature_type: igs
region: SSC
length_bp: 3163
variable_sites: 1329
pi: 0.431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-ycf35_p1	GGGGAATTTCAGGTTCCTCC	GCTTCAGGGTCTTCCTTGTC	55.0	55.0	57.9	58.2	562 bp	0.5	57.4
psbA-ycf35_p2	GGGGAATTTCAGGTTCCTCC	TCAGGGTCTTCCTTGTCCAA	55.0	50.0	57.9	58.2	559 bp	0.5	57.4
psbA-ycf35_p3	GGGGAATTTCAGGTTCCTCC	TTCAGGGTCTTCCTTGTCCA	55.0	50.0	57.9	58.2	560 bp	0.5	57.4
psbA-ycf35_p4	GGGGAATTTCAGGTTCCTCC	TGAGCAACTTCCAAGCGAAA	55.0	45.0	57.9	58.3	287 bp	0.5	56.2
psbA-ycf35_p5	GGGGAATTTCAGGTTCCTCC	TTGAGCAACTTCCAAGCGAA	55.0	45.0	57.9	58.3	288 bp	0.5	56.2