Hotspot

ycf1

IRb · 113351-118978 · 5628 bp

Back to Pleuropterus

Pi 0.000533

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5628
variable_sites: 3
pi: 0.000533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTGGTTCTATGCGTGTTCA	CCGCCCCTTTTCCATTTCAT	45.0	50.0	57.2	58.8	297 bp	1.0	67.7
ycf1_p2	TCACTGGTTCTATGCGTGTT	CCGCCCCTTTTCCATTTCAT	45.0	50.0	57.2	58.8	299 bp	1.0	67.7
ycf1_p3	ACTGGTTCTATGCGTGTTCA	TTCGTTCCATTTCCGTCTGC	45.0	50.0	57.2	58.8	322 bp	1.0	67.3
ycf1_p4	TCACTGGTTCTATGCGTGTT	TTCGTTCCATTTCCGTCTGC	45.0	50.0	57.2	58.8	324 bp	1.0	67.3
ycf1_p5	TCTATGCGTGTTCAAAGACGT	CCGCCCCTTTTCCATTTCAT	42.9	50.0	58.0	58.8	291 bp	1.0	66.2