Hotspot

rps16

LSC · 5266-6398 · 1133 bp

Back to Pleuropterus

Pi 0

MarkerSeek score 58.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1133
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAGCAAAAAGGGTGGGAAT	ACGTACCGACTAAACCAATGA	45.0	42.9	57.3	57.1	1306-1307 bp	1.0	58.9
rps16_p2	AGAGCAAAAAGGGTGGGAAT	TGTACGTACCGACTAAACCA	45.0	45.0	57.3	56.0	1309-1310 bp	1.0	58.1
rps16_p3	AGAGCAAAAAGGGTGGGAAT	CGTACCGACTAAACCAATGACT	45.0	45.5	57.3	58.2	1305-1306 bp	1.0	57.2
rps16_p4	AGAGCAAAAAGGGTGGGAAT	CGTACCGACTAAACCAATGAC	45.0	47.6	57.3	56.7	1305-1306 bp	1.0	56.4
rps16_p5	AGAGCAAAAAGGGTGGGAAT	TGTACGTACCGACTAAACCAA	45.0	42.9	57.3	56.7	1309-1310 bp	1.0	56.3