Hotspot

rps15

SSC · 132480-132743 · 264 bp

Back to Pleuropterus

Pi 0.0265

MarkerSeek score 54.00

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 7
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GCCAGTGACAGCTTCGAATA	TGGATAAATAGCTGCGCACA	50.0	45.0	58.0	57.7	1070-1157 bp	1.0	82.4
rps15_p2	GCCAGTGACAGCTTCGAATA	GGTGGATAAATAGCTGCGCA	50.0	50.0	58.0	58.4	1072-1159 bp	1.0	81.7
rps15_p3	TAAGGTAAATCGGCAGCGAC	TGGATAAATAGCTGCGCACA	50.0	45.0	58.1	57.7	1126-1213 bp	1.0	81.6
rps15_p4	TCTTCTCCGCCAATAATGCC	TGGATAAATAGCTGCGCACA	50.0	45.0	58.0	57.7	1258-1345 bp	1.0	81.4
rps15_p5	TTTATTGCCTCTTCTCCGCC	TGGATAAATAGCTGCGCACA	50.0	45.0	58.0	57.7	1267-1354 bp	1.0	81.4